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Journal|[J]Physical Review B: Condensed Matter & Materials PhysicsVolume 106, Issue 4. 2011. PP 048104
Coarse-grained dynamics of protein synthesis in a cell-free system.
摘要 / Abstract
A complete gene expression reaction is reconstituted in a cell-free system comprising the entire endogenous transcription, translation, as well as mRNA and protein degradation machinery of E. coli. In dissecting the major reaction steps, we derive a coarse-grained enzymatic description of biosynthesis and degradation, from which ten relevant rate constants and concentrations are determined. Governed by zeroth-order degradation, protein expression follows a sharp transition from undetectable levels to constant-rate accumulation, without reaching steady state.
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