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Journal|[J]Physical Review B: Condensed Matter & Materials PhysicsVolume 106, Issue 4. 2011. PP 048104
MT无细胞系统中蛋白质合成的粗粒度动力学。
Coarse-grained dynamics of protein synthesis in a cell-free system.
摘要 / Abstract
MT
一个完整的基因表达反应被重组在一个无细胞系统,包括整个内源性转录、翻译以及mRNA和蛋白质降解机制的大肠杆菌。在剖析主要反应步骤时,我们导出了生物合成和降解的粗粒度酶学描述,从中确定了10个相关的速率常数和浓度。在零级降解的调控下,蛋白质的表达经历了从不可检测到的水平到恒速积累的急剧转变,没有达到稳态。
原文
A complete gene expression reaction is reconstituted in a cell-free system comprising the entire endogenous transcription, translation, as well as mRNA and protein degradation machinery of E. coli. In dissecting the major reaction steps, we derive a coarse-grained enzymatic description of biosynthesis and degradation, from which ten relevant rate constants and concentrations are determined. Governed by zeroth-order degradation, protein expression follows a sharp transition from undetectable levels to constant-rate accumulation, without reaching steady state.
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